blocking buffer Search Results


elisa buffer  (Chondrex Inc)
96
Chondrex Inc elisa buffer
Elisa Buffer, supplied by Chondrex Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/elisa buffer/product/Chondrex Inc
Average 96 stars, based on 1 article reviews
elisa buffer - by Bioz Stars, 2026-04
96/100 stars
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intercept protein free blocking buffer  (LI-COR)
98
LI-COR intercept protein free blocking buffer
Intercept Protein Free Blocking Buffer, supplied by LI-COR, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 98 stars, based on 1 article reviews
intercept protein free blocking buffer - by Bioz Stars, 2026-04
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blocking buffer  (LI-COR)
98
LI-COR blocking buffer
Blocking Buffer, supplied by LI-COR, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/blocking buffer/product/LI-COR
Average 98 stars, based on 1 article reviews
blocking buffer - by Bioz Stars, 2026-04
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pbs blocking buffer  (LI-COR)
99
LI-COR pbs blocking buffer
Procaspase 3 levels indicate lack of curcumin-induced apoptosis at the low, plasma level-informed curcumin concentration. (A) A549, H460, Caco-2, and HT29 cells treated with control, curcumin (4–50 µg/mL) for 24h, or staurosporine (2 µM, positive control) for 4h. Cells were washed with <t>PBS,</t> lysed, and processed for Western blotting as described in Materials and Methods. Each lane was loaded with 40 µg of total <t>protein.</t> <t>Membranes</t> were probed with primary antibodies against caspase 3 and either β-actin or tubulin (loading control), followed by IRDye 680 and 800-conjugated secondary antibodies. Blots were imaged using the ChemiDoc ™ platform. (B) Quantification of procaspase 3 band intensity was normalized to β-actin and expressed relative to the control for each cell line. Dot plots represent individual values from 3 biological replicates per cell line, with additional technical replicates included for A549 and HT29 (total n = 7), and for H460 and Caco-2 (total n = 4). Horizontal lines indicate the group mean and error bars represent standard deviation. Statistical analysis was performed using one-way ANOVA followed by Dunnett’s post hoc test. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001; ns = not significant. β-actin (ab8226) was used as the primary loading control following discontinuation of the tubulin antibody (ab59680); earlier tubulin-normalized blots are presented in the Supplementary Material .
Pbs Blocking Buffer, supplied by LI-COR, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pbs blocking buffer/product/LI-COR
Average 99 stars, based on 1 article reviews
pbs blocking buffer - by Bioz Stars, 2026-04
99/100 stars
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li cor blocking buffer  (LI-COR)
99
LI-COR li cor blocking buffer
Procaspase 3 levels indicate lack of curcumin-induced apoptosis at the low, plasma level-informed curcumin concentration. (A) A549, H460, Caco-2, and HT29 cells treated with control, curcumin (4–50 µg/mL) for 24h, or staurosporine (2 µM, positive control) for 4h. Cells were washed with <t>PBS,</t> lysed, and processed for Western blotting as described in Materials and Methods. Each lane was loaded with 40 µg of total <t>protein.</t> <t>Membranes</t> were probed with primary antibodies against caspase 3 and either β-actin or tubulin (loading control), followed by IRDye 680 and 800-conjugated secondary antibodies. Blots were imaged using the ChemiDoc ™ platform. (B) Quantification of procaspase 3 band intensity was normalized to β-actin and expressed relative to the control for each cell line. Dot plots represent individual values from 3 biological replicates per cell line, with additional technical replicates included for A549 and HT29 (total n = 7), and for H460 and Caco-2 (total n = 4). Horizontal lines indicate the group mean and error bars represent standard deviation. Statistical analysis was performed using one-way ANOVA followed by Dunnett’s post hoc test. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001; ns = not significant. β-actin (ab8226) was used as the primary loading control following discontinuation of the tubulin antibody (ab59680); earlier tubulin-normalized blots are presented in the Supplementary Material .
Li Cor Blocking Buffer, supplied by LI-COR, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/li cor blocking buffer/product/LI-COR
Average 99 stars, based on 1 article reviews
li cor blocking buffer - by Bioz Stars, 2026-04
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tbs intercept blocking buffer  (LI-COR)
98
LI-COR tbs intercept blocking buffer
Procaspase 3 levels indicate lack of curcumin-induced apoptosis at the low, plasma level-informed curcumin concentration. (A) A549, H460, Caco-2, and HT29 cells treated with control, curcumin (4–50 µg/mL) for 24h, or staurosporine (2 µM, positive control) for 4h. Cells were washed with <t>PBS,</t> lysed, and processed for Western blotting as described in Materials and Methods. Each lane was loaded with 40 µg of total <t>protein.</t> <t>Membranes</t> were probed with primary antibodies against caspase 3 and either β-actin or tubulin (loading control), followed by IRDye 680 and 800-conjugated secondary antibodies. Blots were imaged using the ChemiDoc ™ platform. (B) Quantification of procaspase 3 band intensity was normalized to β-actin and expressed relative to the control for each cell line. Dot plots represent individual values from 3 biological replicates per cell line, with additional technical replicates included for A549 and HT29 (total n = 7), and for H460 and Caco-2 (total n = 4). Horizontal lines indicate the group mean and error bars represent standard deviation. Statistical analysis was performed using one-way ANOVA followed by Dunnett’s post hoc test. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001; ns = not significant. β-actin (ab8226) was used as the primary loading control following discontinuation of the tubulin antibody (ab59680); earlier tubulin-normalized blots are presented in the Supplementary Material .
Tbs Intercept Blocking Buffer, supplied by LI-COR, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/tbs intercept blocking buffer/product/LI-COR
Average 98 stars, based on 1 article reviews
tbs intercept blocking buffer - by Bioz Stars, 2026-04
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intercept tbs blocking buffer  (LI-COR)
98
LI-COR intercept tbs blocking buffer
Procaspase 3 levels indicate lack of curcumin-induced apoptosis at the low, plasma level-informed curcumin concentration. (A) A549, H460, Caco-2, and HT29 cells treated with control, curcumin (4–50 µg/mL) for 24h, or staurosporine (2 µM, positive control) for 4h. Cells were washed with <t>PBS,</t> lysed, and processed for Western blotting as described in Materials and Methods. Each lane was loaded with 40 µg of total <t>protein.</t> <t>Membranes</t> were probed with primary antibodies against caspase 3 and either β-actin or tubulin (loading control), followed by IRDye 680 and 800-conjugated secondary antibodies. Blots were imaged using the ChemiDoc ™ platform. (B) Quantification of procaspase 3 band intensity was normalized to β-actin and expressed relative to the control for each cell line. Dot plots represent individual values from 3 biological replicates per cell line, with additional technical replicates included for A549 and HT29 (total n = 7), and for H460 and Caco-2 (total n = 4). Horizontal lines indicate the group mean and error bars represent standard deviation. Statistical analysis was performed using one-way ANOVA followed by Dunnett’s post hoc test. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001; ns = not significant. β-actin (ab8226) was used as the primary loading control following discontinuation of the tubulin antibody (ab59680); earlier tubulin-normalized blots are presented in the Supplementary Material .
Intercept Tbs Blocking Buffer, supplied by LI-COR, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/intercept tbs blocking buffer/product/LI-COR
Average 98 stars, based on 1 article reviews
intercept tbs blocking buffer - by Bioz Stars, 2026-04
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protein free blocking buffer  (LI-COR)
98
LI-COR protein free blocking buffer
Procaspase 3 levels indicate lack of curcumin-induced apoptosis at the low, plasma level-informed curcumin concentration. (A) A549, H460, Caco-2, and HT29 cells treated with control, curcumin (4–50 µg/mL) for 24h, or staurosporine (2 µM, positive control) for 4h. Cells were washed with <t>PBS,</t> lysed, and processed for Western blotting as described in Materials and Methods. Each lane was loaded with 40 µg of total <t>protein.</t> <t>Membranes</t> were probed with primary antibodies against caspase 3 and either β-actin or tubulin (loading control), followed by IRDye 680 and 800-conjugated secondary antibodies. Blots were imaged using the ChemiDoc ™ platform. (B) Quantification of procaspase 3 band intensity was normalized to β-actin and expressed relative to the control for each cell line. Dot plots represent individual values from 3 biological replicates per cell line, with additional technical replicates included for A549 and HT29 (total n = 7), and for H460 and Caco-2 (total n = 4). Horizontal lines indicate the group mean and error bars represent standard deviation. Statistical analysis was performed using one-way ANOVA followed by Dunnett’s post hoc test. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001; ns = not significant. β-actin (ab8226) was used as the primary loading control following discontinuation of the tubulin antibody (ab59680); earlier tubulin-normalized blots are presented in the Supplementary Material .
Protein Free Blocking Buffer, supplied by LI-COR, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/protein free blocking buffer/product/LI-COR
Average 98 stars, based on 1 article reviews
protein free blocking buffer - by Bioz Stars, 2026-04
98/100 stars
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blocking buffer  (Thermo Fisher)
99
Thermo Fisher blocking buffer
Procaspase 3 levels indicate lack of curcumin-induced apoptosis at the low, plasma level-informed curcumin concentration. (A) A549, H460, Caco-2, and HT29 cells treated with control, curcumin (4–50 µg/mL) for 24h, or staurosporine (2 µM, positive control) for 4h. Cells were washed with <t>PBS,</t> lysed, and processed for Western blotting as described in Materials and Methods. Each lane was loaded with 40 µg of total <t>protein.</t> <t>Membranes</t> were probed with primary antibodies against caspase 3 and either β-actin or tubulin (loading control), followed by IRDye 680 and 800-conjugated secondary antibodies. Blots were imaged using the ChemiDoc ™ platform. (B) Quantification of procaspase 3 band intensity was normalized to β-actin and expressed relative to the control for each cell line. Dot plots represent individual values from 3 biological replicates per cell line, with additional technical replicates included for A549 and HT29 (total n = 7), and for H460 and Caco-2 (total n = 4). Horizontal lines indicate the group mean and error bars represent standard deviation. Statistical analysis was performed using one-way ANOVA followed by Dunnett’s post hoc test. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001; ns = not significant. β-actin (ab8226) was used as the primary loading control following discontinuation of the tubulin antibody (ab59680); earlier tubulin-normalized blots are presented in the Supplementary Material .
Blocking Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/blocking buffer/product/Thermo Fisher
Average 99 stars, based on 1 article reviews
blocking buffer - by Bioz Stars, 2026-04
99/100 stars
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casein blocking buffer in pbs  (Thermo Fisher)
97
Thermo Fisher casein blocking buffer in pbs
Procaspase 3 levels indicate lack of curcumin-induced apoptosis at the low, plasma level-informed curcumin concentration. (A) A549, H460, Caco-2, and HT29 cells treated with control, curcumin (4–50 µg/mL) for 24h, or staurosporine (2 µM, positive control) for 4h. Cells were washed with <t>PBS,</t> lysed, and processed for Western blotting as described in Materials and Methods. Each lane was loaded with 40 µg of total <t>protein.</t> <t>Membranes</t> were probed with primary antibodies against caspase 3 and either β-actin or tubulin (loading control), followed by IRDye 680 and 800-conjugated secondary antibodies. Blots were imaged using the ChemiDoc ™ platform. (B) Quantification of procaspase 3 band intensity was normalized to β-actin and expressed relative to the control for each cell line. Dot plots represent individual values from 3 biological replicates per cell line, with additional technical replicates included for A549 and HT29 (total n = 7), and for H460 and Caco-2 (total n = 4). Horizontal lines indicate the group mean and error bars represent standard deviation. Statistical analysis was performed using one-way ANOVA followed by Dunnett’s post hoc test. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001; ns = not significant. β-actin (ab8226) was used as the primary loading control following discontinuation of the tubulin antibody (ab59680); earlier tubulin-normalized blots are presented in the Supplementary Material .
Casein Blocking Buffer In Pbs, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/casein blocking buffer in pbs/product/Thermo Fisher
Average 97 stars, based on 1 article reviews
casein blocking buffer in pbs - by Bioz Stars, 2026-04
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li cor intercept pbs blocking buffer  (LI-COR)
99
LI-COR li cor intercept pbs blocking buffer
Procaspase 3 levels indicate lack of curcumin-induced apoptosis at the low, plasma level-informed curcumin concentration. (A) A549, H460, Caco-2, and HT29 cells treated with control, curcumin (4–50 µg/mL) for 24h, or staurosporine (2 µM, positive control) for 4h. Cells were washed with <t>PBS,</t> lysed, and processed for Western blotting as described in Materials and Methods. Each lane was loaded with 40 µg of total <t>protein.</t> <t>Membranes</t> were probed with primary antibodies against caspase 3 and either β-actin or tubulin (loading control), followed by IRDye 680 and 800-conjugated secondary antibodies. Blots were imaged using the ChemiDoc ™ platform. (B) Quantification of procaspase 3 band intensity was normalized to β-actin and expressed relative to the control for each cell line. Dot plots represent individual values from 3 biological replicates per cell line, with additional technical replicates included for A549 and HT29 (total n = 7), and for H460 and Caco-2 (total n = 4). Horizontal lines indicate the group mean and error bars represent standard deviation. Statistical analysis was performed using one-way ANOVA followed by Dunnett’s post hoc test. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001; ns = not significant. β-actin (ab8226) was used as the primary loading control following discontinuation of the tubulin antibody (ab59680); earlier tubulin-normalized blots are presented in the Supplementary Material .
Li Cor Intercept Pbs Blocking Buffer, supplied by LI-COR, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/li cor intercept pbs blocking buffer/product/LI-COR
Average 99 stars, based on 1 article reviews
li cor intercept pbs blocking buffer - by Bioz Stars, 2026-04
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startingblocktm t20 blocking buffer  (Thermo Fisher)
98
Thermo Fisher startingblocktm t20 blocking buffer
Procaspase 3 levels indicate lack of curcumin-induced apoptosis at the low, plasma level-informed curcumin concentration. (A) A549, H460, Caco-2, and HT29 cells treated with control, curcumin (4–50 µg/mL) for 24h, or staurosporine (2 µM, positive control) for 4h. Cells were washed with <t>PBS,</t> lysed, and processed for Western blotting as described in Materials and Methods. Each lane was loaded with 40 µg of total <t>protein.</t> <t>Membranes</t> were probed with primary antibodies against caspase 3 and either β-actin or tubulin (loading control), followed by IRDye 680 and 800-conjugated secondary antibodies. Blots were imaged using the ChemiDoc ™ platform. (B) Quantification of procaspase 3 band intensity was normalized to β-actin and expressed relative to the control for each cell line. Dot plots represent individual values from 3 biological replicates per cell line, with additional technical replicates included for A549 and HT29 (total n = 7), and for H460 and Caco-2 (total n = 4). Horizontal lines indicate the group mean and error bars represent standard deviation. Statistical analysis was performed using one-way ANOVA followed by Dunnett’s post hoc test. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001; ns = not significant. β-actin (ab8226) was used as the primary loading control following discontinuation of the tubulin antibody (ab59680); earlier tubulin-normalized blots are presented in the Supplementary Material .
Startingblocktm T20 Blocking Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/startingblocktm t20 blocking buffer/product/Thermo Fisher
Average 98 stars, based on 1 article reviews
startingblocktm t20 blocking buffer - by Bioz Stars, 2026-04
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Image Search Results


Procaspase 3 levels indicate lack of curcumin-induced apoptosis at the low, plasma level-informed curcumin concentration. (A) A549, H460, Caco-2, and HT29 cells treated with control, curcumin (4–50 µg/mL) for 24h, or staurosporine (2 µM, positive control) for 4h. Cells were washed with PBS, lysed, and processed for Western blotting as described in Materials and Methods. Each lane was loaded with 40 µg of total protein. Membranes were probed with primary antibodies against caspase 3 and either β-actin or tubulin (loading control), followed by IRDye 680 and 800-conjugated secondary antibodies. Blots were imaged using the ChemiDoc ™ platform. (B) Quantification of procaspase 3 band intensity was normalized to β-actin and expressed relative to the control for each cell line. Dot plots represent individual values from 3 biological replicates per cell line, with additional technical replicates included for A549 and HT29 (total n = 7), and for H460 and Caco-2 (total n = 4). Horizontal lines indicate the group mean and error bars represent standard deviation. Statistical analysis was performed using one-way ANOVA followed by Dunnett’s post hoc test. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001; ns = not significant. β-actin (ab8226) was used as the primary loading control following discontinuation of the tubulin antibody (ab59680); earlier tubulin-normalized blots are presented in the Supplementary Material .

Journal: Pharmaceutical Biology

Article Title: Low, plasma level‑informed native curcumin concentrations fail to induce cell death in human lung and colorectal cancer cells

doi: 10.1080/13880209.2026.2640678

Figure Lengend Snippet: Procaspase 3 levels indicate lack of curcumin-induced apoptosis at the low, plasma level-informed curcumin concentration. (A) A549, H460, Caco-2, and HT29 cells treated with control, curcumin (4–50 µg/mL) for 24h, or staurosporine (2 µM, positive control) for 4h. Cells were washed with PBS, lysed, and processed for Western blotting as described in Materials and Methods. Each lane was loaded with 40 µg of total protein. Membranes were probed with primary antibodies against caspase 3 and either β-actin or tubulin (loading control), followed by IRDye 680 and 800-conjugated secondary antibodies. Blots were imaged using the ChemiDoc ™ platform. (B) Quantification of procaspase 3 band intensity was normalized to β-actin and expressed relative to the control for each cell line. Dot plots represent individual values from 3 biological replicates per cell line, with additional technical replicates included for A549 and HT29 (total n = 7), and for H460 and Caco-2 (total n = 4). Horizontal lines indicate the group mean and error bars represent standard deviation. Statistical analysis was performed using one-way ANOVA followed by Dunnett’s post hoc test. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001; ns = not significant. β-actin (ab8226) was used as the primary loading control following discontinuation of the tubulin antibody (ab59680); earlier tubulin-normalized blots are presented in the Supplementary Material .

Article Snippet: Complete Mini Protease Inhibitor Cocktail Tablet was obtained from Roche; the BCA Protein Assay Kit from Thermo Fisher Scientific; 4–20% SDS-PAGE Gels from Bio-Rad; PVDF membranes from Merck; and Intercept TM (PBS) Blocking Buffer from LI-COR Biosciences.

Techniques: Clinical Proteomics, Concentration Assay, Control, Positive Control, Western Blot, Standard Deviation